Skip to main content
. Author manuscript; available in PMC: 2013 Jul 9.
Published in final edited form as: Methods Enzymol. 2009;457:255–273. doi: 10.1016/S0076-6879(09)05014-9

Figure 14.4.

Figure 14.4

Configuration of mitochondrial assay system. Mitochondria preparation is suspended in stirred cuvette. Excitation source is light emitting diode (LED) and BP 510/80 Fluorescence filter. Mitochondrial swelling isdetected by 90 degree scattered light at photomultipler tube (PMT).TMRM fluorescence is detected by Spectrophotometer1, and oxygen is detected using a fiber–optic sensor inside of the mitochondrial solution by Spectrophotometer 2. Signals are logged by analog to digital converter (ADC) in a PC.