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. Author manuscript; available in PMC: 2013 Jul 9.
Published in final edited form as: Methods Enzymol. 2009;457:255–273. doi: 10.1016/S0076-6879(09)05014-9

Table 14.1.

Prediction of mitochondria targeting for PP2Cm

MitoProt II (http://ihg.gsf.de/ihg/mintoprot.html):
Net charge of query sequence :−4
Analyzed region : 29
Number of basic residues in targeting sequence : 5
Number of acidic residues in targeting sequence : 0
Cleavage site : 30
Cleaved sequence : MSTAALITLVRSGGNQVRRVLLSSRLLQ
Probability of export to mitochondria : 0.9738
iPSORT Prediction (http://he.ims.u-tokyo.ac.jp/IPSORT/):
Values used for reasoning
Node Answer View Substring Value(s)
1. Signal peptide? No Average hydropathy (KYTJ820101) [6,20] −0.453333 (≥0.953? No)
2. Mitochondrial? Yes Average net charge (KLEP 840101) [1,30] 0.133333(≥0.083? Yes)
Indexing:All
Pattern:221121122(ins/del≤3) [1,30] MSTAALITLVRSGGN QV-R-RRVL LSSRLLQD 222222122212000 22-1-1122 22212220 221121122