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. 2013 Apr 22;5(4):1131–1142. doi: 10.3390/v5041131

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Characterization of Fab binding with LMP1-TES1 domain. (A) ELISA showed that htesFab bound LMP1-TES domain in native confirmation (p < 0.05); (B) Immunoprecipitation analysis for the detection of LMP1 protein. LMP1 was 53 kDa; Line 1: HNE2 cells; line 2: HNE2-LMP1 cells; line 3: unrelated Fab fragment (C); Immunofluorescence analysis showed that htesFab labeled LMP1 in the intracellular and plasma membranes in HNE2-LMP1 cells (green), cell nuclei were stained with DAPI (blue) (×200, Olympus digital camera); (D) FACS analysis showed the binding of htesFab to HNE2-LMP1(40.35%) and HNE2 cells(4.15%) (blue dots). Background staining was obtained by PBS.