Table 3.
MS/MS settings for microcystin (MC) and nodularin (NOD) analysis.
| Compound | Retention time (min) | Precursor ion (m/z) | Fragmentor (V) | Quantifier ion (m/z) | CE 1 quantifier (V) | Qualifier ion (m/z) | CE 1 qualifier (V) | Ratio 2 (%) |
|---|---|---|---|---|---|---|---|---|
| dm-7-MC-RR | 6.93 | 512.8 | 135 | 135.1 | 26 | 70.1 | 85 | 1.2 |
| MC-RR | 7.62 | 519.8 | 151 | 135.1 | 30 | 70.1 | 75 | 2.7 |
| NOD | 8.03 | 825.5 | 220 | 135.1 | 70 | 70.1 | 95 | 44.2 |
| MC-YR | 8.16 | 523.3 | 102 | 911.5 | 5 | 135.1 | 6 | 103.6 |
| dm-7-MC-LR | 8.21 | 491.3 | 88 | 847.6 | 5 | 135.1 | 6 | 84.0 |
| MC-LR | 8.24 | 498.3 | 88 | 135.1 | 6 | 482.3 | 6 | 56.7 |
| MC-LY | 9.67 | 868.4 | 170 | 163.0 | 35 | 136.1 | 75 | 29.0 |
| MC-LW | 10.22 | 891.5 | 146 | 163.0 | 31 | 159.0 | 75 | 26.9 |
| MC-LF | 10.47 | 852.5 | 140 | 163.0 | 31 | 120.1 | 79 | 39.1 |
1 collision energy, 2 ratio between abundance of the qualifier and quantifier ion.