Figure 1.

Generation and phenotypic analysis of TYLCSV-infected 2IRGFP N. benthamiana transgenic plants. a. Construct 2IRGFP contains a direct repeat of the TYLCSV IR encompassing a GFP expression cassette that contains the 35S CaMV promoter (P35S), the complete ORF of GFP and the NOS terminator (Ter). During TYLCSV infection, the viral Rep protein specifically recognizes the IRs flanking the cassette, and mGFP replicons are generated (EM), which in turn leads to a strong over-expression of the GFP transgene and the subsequent accumulation of the fluorescent protein. b. Evolution of virus replication-associated phenotype (RAP) in infected 2IRGFP plants at different days post-infection (dpi). A representative photograph of each RAP phenotype showing the extension and intensity of GFP expression is displayed.