Table 3.
In vitro antiprotozoal activity of fractions obtained from the ethyl acetate crude extract of B. bifurcata. Data shown are means of two independent assays, which varied <±50%. SI: selectivity index; ratio of cytotoxic activity on L6 cells to antiprotozoal activity measured against a T. brucei rhodesiense, b T. cruzi and c P. falciparum. * Data from [6].
| Antiprotozoal activity | Cytotoxic activity | ||||||
|---|---|---|---|---|---|---|---|
| IC50 (µg/mL) | IC50 (µg/mL) | SI | |||||
| T. brucei rhodesiense | T. cruzi | P. falciparum | L6 cells | SI a | SI b | SI c | |
| Ethyl acetate extract | 0.5 | 4.1 * | >5 * | 6.2 * | 12.4 | 1.5 * | >1 * |
| Fractions | |||||||
| Fraction 1 | 12.9 | 32.4 | 8.5 | 40.2 | 3.1 | 1.2 | 4.7 |
| Fraction 2 | 0.5 | 9.7 | 3.8 | 7.4 | 15.4 | 0.8 | 1.9 |
| Fraction 3 | 4.3 | 11.7 | 3.7 | 16.9 | 3.9 | 1.4 | 4.6 |
| Fraction 4 | 14.7 | 25.0 | 3.0 | 55.1 | 3.7 | 2.2 | 18.4 |
| Fraction 5 | 14.1 | 32.1 | 4.8 | 38.7 | 2.7 | 1.2 | 8.0 |
| Standard drugs | |||||||
| Melarsoprol | 0.004 | ||||||
| Benznidazole | 0.536 | ||||||
| Chloroquine | 0.069 | ||||||
| Artemisinin | 0.002 | ||||||
| Podophyllotoxin | 0.007 | ||||||