Figure 3.

Comparison of the “neurotoxic fraction” (FrIII) from two populations of the sea anemone B. caissarum: southeastern coast of Brazil and Saint Peter and Saint Paul Archipelago. The black continuous line represents the rp-HPLC profile of FrIII from the Southeastern coast population. Labeled peaks were the previously characterized neurotoxins BcIII [29] and BcIV [35]. Red dotted line is the FrIII chromatographic profile of the SPSPA population. “Neurotoxic fractions” were submitted to rp-HPLC chromatography using a semi-preparative CAPCELL PAK C-18 column (1 × 25 cm, Shiseido Corp.), and their components were eluted with a linear gradient from 10% to 60% of acetonitrile containing 0.1% TFA, as described in the Experimental Section.