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. 2013 Mar 6;11(3):655–679. doi: 10.3390/md11030655

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Phylogenetic analysis and sequence alignment. (A) Amino acid sequence of BcsTx1 and BcsTx2 and multiple sequence alignment with the other members of type 1 sea anemone toxins. Alignment was based on the cysteine residues. Disulfide bridge pattern are indicated. Amino acid identities (black boxes) and similarities (grey boxes) are shown. (B) The phylogenetic tree of type 1 sea anemone K_V-toxins was constructed with the Neighbor-joining algorithm of MEGA 4.0. The consensus tree shown supports the suggested division of sea anemone type 1 into two different subtypes. The scale bar shows amino acid substitution rates. Only the mature region of the sequences reported as FC850067, FK724096, FK755121 and FK747792 were used in the analysis.