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. 2013 Mar 14;11(3):881–895. doi: 10.3390/md11030881

Figure 3.

Figure 3

Investigation of disulfide-mediated dimerisation of 2H12 scFvs. (A) Coomassie blue-stained SDS-PAGE and (B) Immunoblot analysis of disulfide reduced (lanes 2–4) and non-reduced (lanes 5–7) purified 2H12 scFv (lanes 2,5), 2H12 scFv-cys I (3,6) and 2H12 scFv-cys II (4,7). Lane 1: Molecular weight marker. Samples in lanes 2,3,4 were loaded in reducing conditions; samples in lanes 5,6,7 were loaded in non-reducing conditions. Arrows indicate the monomeric scFv; asterisks indicate dimeric scFv molecules.