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Comparison of domoic acid binding of (A) adsorbed and (B) covalently attached, oriented 2H12 scFvs. (A) ■ wildtype scFv on polystyrene; ● scFv-cys I on polystyrene; ▲ scFv-cys II on polystyrene. (B) ● scFv-cys I on maleimide-activated surface; ▲ scFv-cys II on maleimide-activated surface.
