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. 2013 Mar 14;11(3):881–895. doi: 10.3390/md11030881

Figure 6.

Figure 6

Stability of domoic acid binding by immobilised scFv fragments. Immobilised scFvs (black: wildtype 2H12 scFv; red: scFv-cys I; blue: scFv-cys II) were washed for 1 h at room temperature with: (1) Normal wash buffer (PBS containing 0.05% (v/v) Tween 20, pH 7.4); (2) 100 mM phosphate buffer containing 1 M NaCl, pH 7.2; (3) 100 mM acetate buffer, pH 5.0; (4) 100 mM acetate buffer, pH 3.6. Upon completion of the domoic acid-binding assay, DA binding was expressed as a percentage of the binding measured after washing with normal washing buffer. The wildtype scFv was immobilised on Maxisorp plates, the cysteine-modified scFvs on maleimide-functionalised plates.