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Scheme demonstrating the preparation of sponge skeletons, including skeleton isolation by H2O or TE100, MeOH-based extraction of the skeletons and extraction of the chitin-based scaffold by NaOH (top: A. cavernicola, scale bar: 100 µm; bottom: I. basta, scale bar: 100 µm). The treatment procedures are described in Section 3.
