Changes in myelin protein levels with post-mortem delay. Post-mortem delay was simulated by incubation of homogenates of adjacent samples of cortex at room temperature (RT) and 4°C for varying lengths of time. The protein levels of myelin-associated glycoprotein (MAG), myelin-basic protein (MBP), and proteolipid protein (PLP) were measured by enzyme-linked immunosorbent assay (ELISA). (A) The protein levels of MBP fell progressively with increasing incubation time, both at room temperature (r=−0.775, P=0.0408) and at 4°C (r=−0.951, P=0.0495). (B) The protein levels of MAG and (C) PLP did not alter significantly over 72 hours.