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. 2013 Apr 24;33(7):1127–1137. doi: 10.1038/jcbfm.2013.64

Figure 1.

Figure 1

Detection of lectin storage and microglia activation in the central nervous system (CNS) of FVB/Twitcher (Twi) mice. (A) Histochemistry using biotinylated lectins (detecting glycolipid storage and globoid cells) and (B, C) immunohistochemistry analyses using antibodies to CD68 and Iba1 (detecting macrophages and microglia) were performed on selected forebrain, cerebellar (Cb), and spinal cord (SC) tissue slices from PND30 and PND40 WT and Twi mice. Qualitative (representative pictures showing lectin-, CD68- and Iba-1- positive staining in the different CNS regions are shown; scale bars: 500 μm) and quantitative analysis (quantification of immunopositive areas) indicated an age- and region-dependent increase of tissue storage (globoid cells; A) and activated macrophages/microglia (B, C) in Twi mice as compared with WT littermates. Data represent the mean±s.e.m., n=3 mice/group, 2 slices/region, 4 pictures/slice. Two-way analysis of variance followed by Bonferroni post test. Data in A: P< 0.05 when comparing Twi versus WT at PND30 and PND40 for Forebrain, Cb and SC (not indicated in the graph); *P⩽0.05 versus Twi PND30. #P⩽0.05 versus PND30 Twi Forebrain and SC. Data in B: at PND30 (Cb and SC) and PND40 (Forebrain, Cb and SC) P⩽0.05 when comparing Twi versus WT (not indicated in the graph); *P⩽0.05, ***P⩽0.001 versus PND30, #P⩽0.05 versus PND30 Twi Forebrain. Data in C: at PND30 and PND40 (Forebrain, Cb and SC) P⩽0.01 when comparing Twi versus WT (not indicated in the graph), *P⩽0.05, **P⩽0.01 versus PND30.