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. 2013 Apr 24;33(7):1127–1137. doi: 10.1038/jcbfm.2013.64

Figure 2.

Figure 2

Astrogliosis in the central nervous system (CNS) of FVB/Twitcher (Twi) mice. Immunohistochemistry analysis using anti-glial fibrillary acidic protein (GFAP) antibody was performed on selected Forebrain (WM, white matter; GM, gray matter), cerebellar (Cb), and spinal cord (SC) tissue slices from PND30 and PND40 wild-type (WT) and Twi mice. (A-B) Qualitative and quantitative analyses indicated an age- and region-dependent increase of GFAP staining in Twi mice as compared with WT littermates. Representative pictures showing GFAP-positive cells in the different CNS regions are shown. Data represent the mean ±s.e.m.; n=3 mice/group, 2 slices/region, 4 pictures/slice. Two-way analysis of variance followed by Bonferroni post test. At both ages (PND30 and PND40) and in all CNS regions (Forebrain, Cb, and SC) P⩽0.001 when comparing Twi versus WT (not indicated in the graph); *P⩽0.05, **P⩽0.01, ***P⩽0.001 versus PND30. #P⩽0.05 versus Twi forebrain and SC. (C) Representative images from PND40 SC tissue slices showing increased density of GFAP-positive cells with thick processes typical of activated/reactive astrocytes. Scale bars: 500 μm (A), 200 μm (C).