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. Author manuscript; available in PMC: 2013 Nov 30.
Published in final edited form as: Cell Physiol Biochem. 2012 Nov 30;30(6):1444–1455. doi: 10.1159/000343332

Fig. 3.

Fig. 3

Effect of PSD-95 on pHi changes mediated by NBCn1 in HEK 293 cells. A) Representative pHi traces in HEK 293 cells expressing NBCn1 and either PSD-95 or none. Cells were superfused with HCO3−/CO2-free HEPES solution and then with a solution equilibrated with 33 mM HCO3−, 5% CO2. Measurements were done at 37°C. B) Mean rate of pHi recovery from CO2-induced acidification. The rate (dpHi/dt; pH units per second) was calculated from a linear regression line during the first 30 sec of recovery (n = 8 controls, 21 NBCn1 cells, and 20 NBCn1/PSD-95 cells in three independent coverslips). **: significant compared to controls (p < 0.05). n.s.: not significant (p > 0.05).