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. Author manuscript; available in PMC: 2013 Jul 9.
Published in final edited form as: Am J Hypertens. 2009 Apr 16;22(7):770–776. doi: 10.1038/ajh.2009.69

Figure 1.

Figure 1

Insulin receptor expression in HEK293 cells. Cell lysate proteins (50 μg) from RPT cells from WKY rats (lanes 1 and 3) and HEK293 cells (lanes 2 and 4) were subjected to immunoblotting with anti-insulin receptor antibody (1:250). In HEK293 cells and RPT cells from WKY rats, the 95-kDa band was no longer visible when the antibody was preadsorbed with the immunizing peptide (1:10 wt/wt incubation for 12 h). The molecular sizes are indicated. RPT, renal proximal tubule; WKY, Wistar-Kyoto.