Figure 6.

Bcl-xL upregulation is required for GAPDH-dependent protection from CICD. (a) Mock and GAPDH-V5-overexpressing HeLa cells were treated with or without ABT-737 (10 μM)+Q-VD-OPh (20 μM). Inhibitors were added 30 min before a 12-Gy irradiation step and replaced periodically at 48-h intervals for 10 days. Colonies were stained and quantified as described in Figure 1d. (b) Mock or GAPDH-V5-expressing cells were transiently transfected with a control vector (pcDNA₃) or a vector encoding Bcl-xL. After 48 h, the cells were treated with or without 20 μM Q-VD-OPh, and then irradiated (12 Gy). Colonies were stained 18 days later. The quantifications are the means of 3–5 independent experiments. ***P<0.005, *P<0.05. n.s., not significant