Figure 2.

ZNF313 stimulates apoptosis and cell cycle progression. (a) ZNF313 depletion attenuates proapoptotic effect of XAF1. HCT116 cells transfected with WT-XAF1 and/or siZNF313 were exposed to TRAIL (10 ng/ml), etoposide (10 μM) and 5-FU (10 μM) for 48 h and apoptotic sub-G1 fraction was measured by flow cytometry. Data represent means of triplicate assays (bars, S.D.) (*P<0.05). (b) ZNF313 effect on XAF1-induced apoptosis. (c) ZNF313 effect on basal and 5-FU-induced apoptosis. (d) ZNF313 stimulation of a G1-to-S transition of the cell cycle and rescue of siZNF313-induced G1 arrest by co-transfection of WT-ZNF313. (e) ZNF313 effect on cellular response to genotoxic stresses. HCT116 cells transfected with siZNF313 or WT-ZNF313 were exposed to various genotoxic stresses. The G1 arrest and apoptotic response of the cells were determined by flow cytometry. (f) Effect of ZNF313 on colony-forming ability of HCT116 cells (**P<0.01). (g) Effect of ZNF313 on xenograft tumor growth of HCT116 (*P<0.05). DMSO, dimethylsulfoxide; 5-FU, 5-fluorouracil; HA, hemagglutinin; IR, irradiation; PARP, poly (ADP-ribose) polymerase; WT, wild-type