Figure 3.

Detection of HERV-K transcripts in four prostate cancer cell lines. RT-PCR was performed to detect viral sense strand transcripts specifically, and the products were resolved by electrophoresis. (A) RT-PCR products from a primer pair designed to detect unspliced viral RNAs (unspliced). (B) RT-PCR products from primers designed to detect RNAs spliced at the conventional env mRNA splice junction (1×-env). Genomic positions of the primers are shown in Figure 2. Parallel controls were performed without reverse transcriptase (−RT) and with no added template (NTC, no template control) as controls to exclude DNA contamination. DNA size markers are shown on the left.