Figure 6.

Reverse transcriptase-PCR analysis of HERV-K transcripts in LNCaP cells. Strand-specific, RT-PCR was performed on RNA isolated from LNCaP cells for five different positions in the HERV-K genome using the primer pairs identified in Figure 2, and the products were resolved by electrophoresis. Viral strand indicates RT-PCR products that originated from viral sense (+) or antisense (−) RNAs. Parallel controls were performed without reverse transcriptase (−RT) and without adding any RNA template (NTC, no template control) to exclude DNA contamination. DNA size markers are shown on the left.