Table 4.
Human endogenous retrovirus K proviruses detected in LNCaP cells by sequencing plasmid subclones.
| Genomic locus | Viral strand | PCR fragments (amplicons)a |
||||
|---|---|---|---|---|---|---|
| LTR | pro | env-1 | env-2 | env-3 | ||
| K(I) (3q21.2) | Rev | 1/8 | 2/9 | 6/8 | –b | 5/7c |
| Fwd | – | – | – | – | – | |
| K106 (3q13.2) | Rev | – | 1/9 | – | 3/9c | 1/7 |
| Fwd | – | – | – | – | – | |
| K50F (19q13.12) | Rev | – | – | 2/8 | 5/9 | – |
| Fwd | – | – | – | – | – | |
| Fragment 11q12.3 | Rev | – | – | – | 1/9 | 1/7 |
| Fwd | – | – | – | – | – | |
| K118 (3q12.3) | Rev | – | – | – | – | – |
| Fwd | – | – | 10/10c | 10/10 | 7/7 | |
| Fragment 7q34 | Rev | – | 6/9 | – | – | – |
| Fwd | – | – | – | – | – | |
| Solo LTRs (multiple loci) | Rev | 7d/8c | – | – | – | – |
| Fwd | 10/10c | – | – | – | – | |
aNumerators are the number of clones detected from the individual locus indicated, and denominators are the number of clones sequenced. Fwd and Rev indicate whether the RNA matched the forward (sense) or reverse (antisense) strand of the viral DNA genome.
b–Indicates not detected.
cAmbiguous amplicon.
dIn this sample 2 sequences may belong to LTRs of full-length HERV-Ks or to solo LTRs but the sequence is ambiguous.