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. Author manuscript; available in PMC: 2013 Jul 9.
Published in final edited form as: Science. 2011 Nov 10;334(6061):1420–1424. doi: 10.1126/science.1213214

Fig. 2. Organoid cultures of Hopx-labeled cells.

Fig. 2

(A) X-gal stained organoids from HopxLacZ/+ mice after 9 days of crypt culture. (B) Time course of the percentage of LacZ-positive organoids derived from HopxLacZ/+ mice. Two hundred organoids from three different HopxLacZ/+ mice were analyzed (error bars: 1 s.d.). (C) Examples of crypt organoids from HopxLacZ/+ mice stained with X-gal. (D) Examples of crypt organoids from HopxERCre/+;R26LacZ/+ mice (tamoxifen pulse for 12h on day 0.5 of culture). X-gal stained images are shown with a 14 day eosin counter-stained image. Scale bars: 50 μm (A) and 20 μm (C and D).