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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Stem Cell Res. 2013 Apr 3;11(1):587–600. doi: 10.1016/j.scr.2013.03.004

Figure 1.

Figure 1

Radial glia cells generated from hESC (hESC-RG cells). (A) Experimental design of the time points at which the experiments were performed; DIV43 was a starting point for further experiments that were done at 2DIV*, 7DIV*, 21DIV* after that. (B) Neural tube-like structures labeled with Pax6 at 21 DIV. (C) These cells express neural progenitor markers, Sox1 and Nestin, as well as a forebrain marker Foxg1 in (D) vimentin+ radial glia and in (E) β-III-tubulin+ young neurons. (F) Cell proliferation seen with BrdU and Ki67 at 72DIV. (G) Histogram showing that the majority of cells at 72 DIV were labeled with Ki67 whereas a 25% were both BrdU+ and Ki67. (H) The percentage of LeX+ cells at three time points in culture. (I) Flow cytometry analysis: majority of cells were LeX+ (empty); isotype control antibody was used for background determination (solid). The % of Max is the number of cells in each bin divided by the number of cells in the bin that contains the largest number of cells (FlowJo software). (J,K) Quantitative RT-PCR analysis showing the transcriptional profiling of neural and rosto-caudal markers at 21 DIV. Fetal human cerebral cortex (FH-CX) and ganglionic eminence (FH-GE) were used as a positive control. Insets provide higher magnification of the double-labeled cells. BB- bisbenzamide stained cell nuclei. BrdU/Ki67- cells in phase S calculated as a percentage of BrdU+ from all Ki67+ cells. Ki67/BB- Ki67+ calculated as a percentage from all cells labeled with nuclear stain (BB). Scale bars: 50μm, 10μm (inset).