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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Stem Cell Res. 2013 Apr 3;11(1):587–600. doi: 10.1016/j.scr.2013.03.004

Figure 3.

Figure 3

Sorted hESC-RG cells are multipotent in vitro. (A) BrdU incorporation after 2 DIV* in proliferation medium. (B–F) In differentiation medium hESC-RG cells generate mainly mature-looking GFAP+ astrocytes (B), neurons labeled with β-III-tubulin (C), DCX+ (D) or MAP2 antibody (E), and rarely PDGFR-α+ oligodendrocyte progenitors (F). (G) Quantification of hESC-RG progeny after 7 DIV* in differentiation medium. Data represent three independent experiments. ***p < 0.0001. Nuclei were stained with bisbenzamide (Blue). Scale bars: 50μm, 10μm.