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Effects of AIMs on NF-κB activity and IκBα phosphorylation. (a) Immunocytochemical analysis of NF-κB (p65) localization in HeLa cells. Cells were pretreated with AIMs (400 μg/mL) or 0.1% DMSO (vehicle control) for 24 h and then treated with TNF (10 ng/mL) for 30 min (×400; the length of scale bar, 50 μm). (b) Cells were pretreated with AIMs (400 μg/mL) for 1 h and then treated with TNF (10 ng/mL) for the indicated times. Data were representative of two independent experiments.
