Figure 5.

Effects of AG on expression of adipogenic signal genes. (a) Real-time PCR analysis of epididymal adipose tissue from experimental mice shows the expression of adipogenesis-related transcription. Data were normalized to the GAPDH mRNA levels and then compared to CON group measurements, which were assigned a value of 1.0. (b) Protein levels of PPARγ, C/EBPα, and SREBP1c were determined by Western blotting. Each bar represents the mean ± SEM of 3 independent experiments of the RNA and protein samples obtained from 5 mice per each group. ^# P < 0.05 versus CON group. *P < 0.05 versus HF group, **P < 0.01 versus HF group, and ***P < 0.001 versus HF group.