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Hematopoietic stem cell (HSC) niche environment formation in GFP-iPSC–derived teratomas. (a) Immunostaining of CD45+ cells in G-iPSC–derived teratomas. Bar, 75 μm. (b) Flow cytometric analysis of iPSC-derived GFP+ and GFP− CD45+ cells and KSL cells in bone marrow (BM) and teratomas (TR). KSL cells in teratomas ranging from 93 to 620 cells. DAPI, 4′,6-diamidino-2-phenylindole; GFP, green fluorescent protein; G-iPSC, GFP transgenic mice-induced pluripotent stem cell.
