Fig. 6.

RhoA activity is required for the reversal of migration triggered by Slit-2. (A,B) Images of migrating OECs before and after exposure to a Slit-2 gradient without (A) or with (B) toxin-B (10 ng/ml) incubation. White arrowheads and arrows indicate the direction of micropipette and the leading front of OECs, respectively. Scale bars: 20 μm. (C,D) Summary of average migration ratios (C) and the percentages of collapsed or reversed cells in total observed cells (D) in response to Slit-2 gradient under various conditions (see text for details). Data are mean + s.e.m.; **P<0.01, Student's t-test.