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Hsp90 and UNC-45 compete for interaction with myosin. (A) UNC-45 interacts with Hsp90. FLAG beads were incubated with suIs2 worm lysate and myosin-containing complex eluted with FLAG peptide. Hsp90 and UNC-45 were determined by immunoblotting with anti-FLAG antibody and anti-Hsp90 antibody. (B) Titration of UNC-45 to a fixed amount of Hsp90 at a fixed concentration of myosin. The purified proteins were detected by Coomassie Blue staining.
