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. 2011 Oct 15;124(20):3515–3524. doi: 10.1242/jcs.091140

Fig. 2.

Fig. 2.

Melusin interacts with FAK and the MAPK scaffold IQGAP1. (A) Western blot analysis of tyrosine phosphorylated proteins in melusin immunocomplexes from MelTG hearts. (B) FAK co-immunoprecipitation revealed by western blots of melusin immunocomplexes isolated from MelTG hearts after AB for 10 minutes or sham operation (n=4 mice/group). Melusin-null hearts were used as negative controls. (C) Coomassie Blue staining of melusin immunoprecipitate from MelTG hearts. The indicated bands were cut out and subjected to mass spectrometry analysis. (D) Western blot of IQGAP1, KSR-1 and paxillin in melusin immunocomplexes isolated from MelTG hearts after AB for 10 minutes or sham operation (n=4 mice/group). (E) Western blot of IQGAP1 and FAK in melusin immunoprecipitate from wild-type (WT) hearts after AB for 10 minutes or sham operation (n=4/group). Melusin-null hearts were used as negative controls. Input: heart total protein extract.