Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 Jul 9.

Published in final edited form as: Breast Cancer Res Treat. 2012 Mar 29;134(3):1041–1055. doi: 10.1007/s10549-012-1992-x

Fig 10 — SLs analogues induce stress response. Immunoblot analysis of MDA-MB-231 cells treated with SL analogues. (A) immunoblot analysis of cells following treatment with ST362 at either 10 or 5 ppm concentration or vehicle alone (-) for the indicated time points. (B) Bar graph showing densitometric quantification of pP38 levels as shown in (A). (C) Immunoblot analysis of cells treated with either vehicle or ST362 (10 ppm) for 1, 4,8 and 24 hrs. Phosophorylation of HSP27 was analyzed. (D) MDA-MB-231 cells were treated with 10 ppm of the Strigolactone analogue, MEB55, or vehicle only for 4 hrs and then harvested and analyzed by SDS-PAGE for protein expression. Levels of the indicated proteins are depicted. (E) Immunoblot analysis of cells treated with ST362 alone or together with the indicated concentrations of SB203580. (F) Immunoblot analysis of cells treated with MEBB55 alone or together with SB203580. Cells were pretreated with SB203580 for 1hour prior to addition of ST362 or MEB55. Cells were treated with ST362 or MEB55 alone or together with SB203580 for 4 hours.

Fig 10 — SLs analogues induce stress response. Immunoblot analysis of MDA-MB-231 cells treated with SL analogues. (A) immunoblot analysis of cells following treatment with ST362 at either 10 or 5 ppm concentration or vehicle alone (-) for the indicated time points. (B) Bar graph showing densitometric quantification of pP38 levels as shown in (A). (C) Immunoblot analysis of cells treated with either vehicle or ST362 (10 ppm) for 1, 4,8 and 24 hrs. Phosophorylation of HSP27 was analyzed. (D) MDA-MB-231 cells were treated with 10 ppm of the Strigolactone analogue, MEB55, or vehicle only for 4 hrs and then harvested and analyzed by SDS-PAGE for protein expression. Levels of the indicated proteins are depicted. (E) Immunoblot analysis of cells treated with ST362 alone or together with the indicated concentrations of SB203580. (F) Immunoblot analysis of cells treated with MEBB55 alone or together with SB203580. Cells were pretreated with SB203580 for 1hour prior to addition of ST362 or MEB55. Cells were treated with ST362 or MEB55 alone or together with SB203580 for 4 hours.