Fig 9.

Effect of SLs on primary MCF-7 mammosphere integrity and viability. MCF7 cells were seeded in MEBM media into low attachment, 96 well plates in duplicate at 3000 cells per well and primary mammospheres left to grow for 7 days. At which time the indicated doses of Strigolactone analogues were added to the media. (A) Representative images were taken of mammospheres after 2 days of Strigolactone treatment. Dissociation is evident after 2 days exposure to SLs. Magnification 100×, Scale bar, 100uM. Insert, zoomed image. (B) Mammospheres numbers (>100μM) following 5 days Strigolactone treatment. Statistical Analysis, two tailed students t-test, p≤0.05 (*), p≤ 0.01 (**), p≤0.001 (***)