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. 2013 May 6;14(1):25–34. doi: 10.1128/jmbe.v14i1.460

TABLE 2.

Outline of activities and desired outcomes over the 15-week semester.

Weeks Activities Desired Outcomes
1–3
  • Learn basic laboratory techniques

  • Learn and practice the scientific method

  • Learn requirements for growth and maintenance of pure cultures

  • Learn basic genetics

  • Learn how to keep a laboratory notebook

  1. Demonstrate proficiency with bacterial cultures.

  2. Describe proP transport system.

  3. Apply hypothesis-driven inquiry.

  4. Practice accurate note-taking.

4–5
  • Perform mutagenesis/select mutants

  • Paper Discussion #1 (Mutations and osmotic regulation)

  • Lab Report #1

  1. Demonstrate ability to select mutants.

  2. Summarize points from review articles.

  3. Write lab report in scientific paper format.

6–11
  • Learn transduction technique and genetic linkage

  • Lab Report #2

  • Paper Discussion #2 (student-selected)

  1. Apply mutation mapping and selection techniques.

  2. Describe transduction as molecular biology tool.

  3. Demonstrate ability to read and discuss primary literature articles.

12–13
  • PCR and gel electrophoresis

  • Identify mutations

  • Lab Report #3

  • Paper Discussion #3

  1. Amplify and sequence proP with PCR.

  2. Demonstrate ability to run an agarose gel.

  3. Utilize online bioinformatic tools.

14–15
  • Informal PowerPoint presentation on preliminary data

  • Poster preparation and presentation

  1. Summarize and interpret a large data set.

  2. Demonstrate effective oral communication of results.

  3. Explain the difference in format between research papers and posters.

  4. Construct a clear poster for public presentation.

  5. Communicate data with scientific community.