Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 May 6;14(1):93–100. doi: 10.1128/jmbe.v14i1.539

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

©2013 Author(s). Published by the American Society for Microbiology.

This is an Open Access article distributed under the terms of the a Creative Commons Attribution – Noncommercial – Share Alike 3.0 Unported License (http://creativecommons.org/licenses/by-nc-sa/3.0/), which permits unrestricted non-commercial use and distribution, provided the original work is properly cited.

PMC Copyright notice

Sophomore-level biology students do not demonstrate a clear understanding of PCR or the difference between DNA replication and expression. Students from a second-year Molecular Biology course (n = 49) predicted the results of their PCR experiment with the open-ended question shown in Appendix 3 (Pre-lab assessment). Categories of student responses are as follows: 1) Gene (DNA) is present whether or not it is being expressed; 2) Amount of gene (DNA) present is proportional to expression level of gene; 3) Gene (DNA) is only present when it is being expressed; 4) Presence of arabinose allows the GFP gene to get added to the E. coli genome; 5) Unable to follow logic.