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. 2013 Jun 3;123(7):3074–3083. doi: 10.1172/JCI69187

Figure 5. Immune tolerization to FVIII prevents bleeding in FVIII-deficient mice.

Figure 5

(A) WT or FVIII-deficient mice were dosed on days 0 and 15 with immunogenic liposomes (Immunogen), STALs, or left untreated. On day 30, mice were reconstituted with rhFVIII at 50 U/kg or saline. FVIII-deficient mice treated with STALs had significantly less blood loss (μl/g) over 20 minutes following tail clip than mice initially treated with immunogenic liposomes. Percentage of bleeding protection (dashed line) represents blood loss of less than 9.9 μl/g as defined by mean + 3 SDs in WT BALB/c mice. (B) FVIII titers in the 3 reconstituted groups demonstrate that bleeding prevention is accompanied by a significant reduction in anti-FVIII antibodies. Data represent mean ± SEM. A 2-tailed Student’s t test was used to establish the level of significance. NS is defined by a P value greater than 0.05.