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. Author manuscript; available in PMC: 2014 Apr 30.
Published in final edited form as: Biochemistry. 2013 Apr 18;52(17):2955–2966. doi: 10.1021/bi400179m

Figure 6.

Figure 6

Inhibition of APE1’s endonuclease activity by E3330 and E3330-amide. A. Oligonucleotide gel-based APE1 endonuclease activity assays. Oligonucleotide gel-based APE1 endonuclease activity assays were performed as described in Experimental Procedures. The upper band (26-mer) represents uncleaved AP oligonucleotide, whereas the lower band (14-mer) is the cleaved oligonucleotide. No inhibition of APE1’s endonuclease activity was observed for concentrations of E3330 less than 160 μM. B. and C. HTS assay for the inhibition of APE1 endonuclease DNA repair activity. When tested for APE1 DNA repair inhibition, the addition of up to 100 μM E3330 (B) or E3330-amide (C) did not result in a decrease in the reaction rate; an indicator of the lack of inhibition.