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. 2013 Jul 9;8(7):e68691. doi: 10.1371/journal.pone.0068691

Figure 2. Role of transcription binding elements GATA, NBRE/Nur77 and SF1 for the activation of the HSD3B2 promoter in NCI-R cells.

Figure 2

(A–C) Promoter activation by GATA and Nur77 was studied in NCI-R cells transiently transfected with −301 bp HSD3B2 promoter luciferase reporter constructs harboring specific binding site mutations. (A) Mutation of the GATA binding site in the −301 HSD3B2 construct showing loss of GATA induced promoter activation. (B) Mutation of the NBRE/Nur77 binding site revealing not only loss in Nur77 but also GATA4/6 induced promoter activation. (C) Mutation of the SF1/LRH binding site showing rather an increase in HSD3B2 promoter activation. Data are expressed as fold over vector control and given as mean±SD of three independent experiments. * p<0.05, ** p<0.01, (a) p = 0.26, (b) p = 0.060.