Fig. 3.

Upregulation and translocation of cryAB and PcryAB to the mitochondria under oxidative stress conditions. A: immunoblots of cryAB and PcryAB along with actin standards in WT mouse adult CM controls or CMs exposed to 100 μM H₂O₂ for 1 h. B: quantification of cryAB and PcryAB levels in adult mouse CMs after exposure to 100 μM H₂O₂ relative to control levels (*P < 0.05). C: immunoblots of cytosolic and organellar subcellular fractions for cryAB and PcryAB in cultured neonatal CMs under control conditions and after exposure to 60 μM H₂O₂. The distribution of VDAC, a mitochondrial marker, and GAPDH, a cytosolic marker, was assessed to monitor the purity of the fractions. D: quantification of the mitochondrial and cytosolic distribution of cryAB and PcryAB in cultured neonatal CMs after exposure to 60 μM H₂O₂ relative to control conditions (#P < 0.05). The band intensities of cryAB organellar levels are expressed in arbitrary units (AU). Values are reported as means ± SE; n = 3. *P < 0.05, mitochondria vs. control cytosol conditions; **P < 0.05, mitochondria vs. stress cytosol conditions.