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. 2013 Jun 5;10:59. doi: 10.1186/1742-4690-10-59

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Copyright © 2013 Manoussaka et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Distribution of SIV envelope antigen and SIV RNA in small intestine. Representative images show (a-c) immunohistochemical staining for anti-SIV envelope (brown cell surface staining) and (d-f) in situ hybridisation for replicating SIV (purple stained cells). Low levels of anti-envelope staining within the lamina propria/villi of Group A animals (a) corresponds to sites of low level viral replication (d, arrows). Higher levels of anti-envelope staining within the follicular marginal zones of Group B animals (b) and follicles of Group C animals (c) also correspond to sites of increased levels of viral replication (e, arrows; f, within oval). Magnification ×100.