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. 2013 Jul 9;8(7):e69304. doi: 10.1371/journal.pone.0069304

Figure 1. Cas family protein alignment and tyrosine phosphorylation.

Figure 1

A. Cas family protein sequences aligned at the PRALINE website. Shown is the region of alignment encompassing human p130CasY249 (LAPGPQDIyDVPPVRGL) indicated by an asterisk and underlined. Numbers above the alignments refer to amino acid number in the NEDD9 human protein sequence, from human NEDD9 residue G144 to A234. The two highlighted tyrosine residues (Y189, Y214) indicate the two NEDD9 residues of interest. Shading indicates the degree of sequence conservation, with red (10) indicating the greatest conservation as shown in the colour gradient at the bottom of the figure. The full sequence alignment is shown in Figure S1. B. Summary of high throughput tandem mass spectrometry studies from phosphosite plus that report detection of phosphorylated NEDD9 Y189, p130Cas Y249 and NEDD9 Y214. Data accessed and analysed as per the materials and methods. Numbers (n) = the number of non-redundant positive records for each site. Pie charts show the representative distributions of the positive records between the different tumour cell types.