Skip to main content
. 2013 Jul 9;8(7):e69304. doi: 10.1371/journal.pone.0069304

Figure 2. NEDD9 mutations do not affect global tyrosine phosphorylation or targeting to focal adhesions.

Figure 2

A. Schematic representation of NEDD9 protein sequence showing the N-terminal SH3 domain, substrate domain containing 13 consensus tyrosine phosphorylation motifs (Y), the serine rich domain (SRR) and the Focal Adhesion Targeting domain (FAT). The two mutated tyrosine residues are indicated in red in the Y189F and Y214F schematic representations. B. Cells transfected with the indicated expression constructs were held in suspension (–) or suspended and then plated onto fibronectin (+). NEDD9 immunoprecipitates were probed with antibodies to phosphotyrosine (P-Tyr) and with anti-NEDD9 antibodies (GFP fusions). C. Cells transfected with GFP vector (A–C), GFP.NEDD9 (D–F), GFP.NEDD9 Y189F (G–I) and GFP.NEDD9 Y214F (J–L). Left hand panels show GFP fluorescence images, middle panels show paxillin immunostaining and merged images are shown on the right. Arrows point to examples of positive focal adhesions. Each image shows a cropped region of one cell.