Table 1. Conservation of tyrosine phosphorylation consensus motifs (-YXXP-) contained in Cas family protein substrate domains.

Residue #		NEDD9	P130Cas	Efs	HEPL
Y92-p	TFGQQKLyQVPNPQA	4/4	3/4	1/3	3/4
Y106-p	AAPRDTIyQVPPSyQ	4/4	–	–	–
Y118-p	SYQNQGIyQVPtGHG	4/4	3/4	3/3	–
Y132-p	GTQEQEVyQVPPSVQ	4/4	3/4	–	–
Y166-p	RTGHGYVyEYPSRYQ	4/4	4/4	3/3	4/4
Y177-p	SRVQKDVyDIPPSHT	4/4	3/4	–	–
Y189-p	SHTTQGVyDIPPSSA	4/4	3/4	–	–
Y214-p	EIKPQGVyDIPPTKG	4/4	–	–	–
Y223-p	IPPTKGVyAIPPSAC	4/4	4/4	3/3	2/4
Y241-p	AGLREKDyDFPPPMR	3/4	–	–	–
Y261-p	DLRPEGVyDIPPTCT	4/4	3/4	3/3	–
Y317-p	VGSQNDAyDVPRGVQ	4/4	3/4	3/3	4/4
Y345-p	PQERDGVyDVPLHNP	4/4	4/4	3/3	–

The amino acid residue number of the 13 NEDD9 YXXP motifs contained in the human NEDD9 substrate domain (aas 59–399) are shown in the first column. The surrounding sequence is shown in the second column. Numbers reflect conservation of the tyrosine in the orthologues examined for each Cas family protein. Human, mouse, rat and chicken orthologues were examined for all family members with the exception of Efs which did not include the chicken orthologue. Cases where no conservation was observed are indicated by a dash (–).