Figure 3.

Repression of UGA recoding by L30 is rescued by SBP2. A. In vitro UGA recoding assays were performed using a luciferase reporter RNA that contains UGA (top panel) or UGU (bottom panel) at position 258 of the coding region, fused to either the PHGPx or TR1 SECIS element as indicated. Translation assays were performed in the presence of increasing amounts of L30 as indicated. The products were analyzed for luciferase activity using a luminometer, and the results are expressed as means ± SEM. Statistical significance is indicated by ** (p < 0.01) and *** (p < 0.001). B. UGA recoding assays with the luc/UGA/TR1 reporter construct were performed with a constant amount of L30 (44 pmol/reaction) and increasing amounts of SBP2 as indicated. The products were analyzed as described in (A).