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REMSA analysis of the RNA-binding activity of L30. A. The 32P-labeled PHGPx SECIS was incubated with increasing amounts of L30 as indicated. The complexes were analyzed by native gel electrophoresis (top panel). The percent SECIS bound at each protein concentration is shown graphically (bottom panel). B. REMSA analysis was performed as described in (A) except that the stem-loop from the L30 pre-mRNA (L30 RNA) was used as the probe.
