Table 1. Gene-specific primers (GSP) for the CCAP receptor in R. prolixus.

A) Oligo name	Oligo Sequence (5′ to 3′)
5′ RACE primers
CCAPR_FOR1	AGCACTGGATAATGGACTGG
CCAPR_FOR2	AGCATTTGCAGATTTATCAGTTG
CCAPR_FOR3	ATCGTCTGGATGCAATTACAAG
CCAPR_FOR4	GGTGGAGATAAGGGAGATGAC
CCAPR_FOR5	CCACGTTTATTCAAAGTCTTGC
3′ RACE primers
CCAPR_REV1	GATTATCTTGGTTACGAATAGTGG
CCAPR_REV2	GGC TAC TGC GAT ATT TGT TTG AG
CCAPR_REV3	TGT CAT CTC CCT TAT CTC CAC C
CCAPR_REV4	TCCATTCAACCGTGATCC
CCAPR_REV5	CCC AAA ACA ATC GCT GC
B) Oligo name	Oligo Sequence (5′ to 3′)
CCAPR specific primer for qPCR
qPCR _CCAPR_F1	GCTTAGCACTGGATAATGGACTG
qPCR _CCAPR_R1	TCAATACGCTGATCAGTCCAACT
Reference genes for qPCR
qPCR _Actin F1	AGAGAAAAGATGACGCAGATAATGT
qPCR _Actin R1	CGGCCAAATCCAATCG
qPCR _RP48_F1	GTGAAACTCAGGAGAAATTGGC
qPCR _RP48_R1	GCATCATCAACATCTCTAATTCCTTG
C) Oligo name	Oligo Sequence (5′ to 3′)
Primers to amplify full ORF
CCAPR_ORF_F1	TTAGCACTGGATAATGGACTGG
CCAPR_ORF_F2	ATGGACTGGGTTATAAGAGATAATTAC
CCAPR_ORF_R1	CTATTCGTAACCAAGATAATCTCTAAATG
CCAPR_ORF_R2	CACTATTCGTAACCAAGATAATCTCTAA
Primers for introduce Kozak sequence
Kozak_CCAPR_ORF_F2	GCCACC ATGGACTGGGTTATAAGAG

(A) Primers used for 5′ and 3′ rapid amplification of cDNA ends (RACE). (B) Transcript specific primers for real time PCR for the spatial expression profile. (C) Primers for cloning the complete open reading frame (ORF), and for introducing a Kozak translation initiation sequence at the 5′UTR.