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There was a titration variance using different primers to target different elements of ssAAV2-EGFP or scAAV2-EGFP. (A) Titration of ssAAV2-EGFP using primers targeting CAG, EGFP, WPRE, and pBGH. (B) Titration of ssAAV2-EGFP using primers targeting CB, EGFP, and pBGH; n=6.