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Comparison of titers by traditional qPCR or qPCR after digestion with SmaI (A) showed qPCR titration of ssAAV2-EGFP using primers targeting CAG and ssAAV2-KS using primers targeting WPRE; (B) showed qPCR titration of scAAV2-EGFP using primers targeting EGFP and pBGH. ** P<0.01; * P< 0.05, n=6.
