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Titration of ssAAV2-EGFP or scAAV2-EGFP by SmaI qPCR with all the different primers. (A) The titers of ssAAV2-EGFP using CAG, EGFP, WPRE and pBGH primers. (B) The titers of scAAV2-EGFP using CB, EGFP and pBGH primers. ** P<0.01; * P<0.05, n=6.