Table 1. Apparent binding affinities of PGC-1α/ERR LBD from MST and ITC.

		MST			ITC
ERR LBD	PGC-1α RID1		K D,1 (nM)	K D,2 (µM)	K D (nM)
ERRα	Wild type		62±6	42	71±6
	L2m		119±9	45	115±7
	L3m		609±54	>290	369±66
	L2mL3m		No binding	No binding	No binding
ERRγ	Wild type		47±7	80	53±3
	L2m		91±6	84	71±6
	L3m		517±45	>284	223±27
	L2mL3m		No binding	No binding	No binding

ITC data determined at 20°C and at pH = 7.5 as described in SI.